Biomedical Sciences

Volume 4, Issue 3, September 2018

  • Affinity Profiles Categorize RNA-Binding Proteins into Distinctive Groups

    Naomi Ueda, Riki Kurokawa

    Issue: Volume 4, Issue 3, September 2018
    Pages: 24-31
    Received: 15 October 2018
    Accepted: 31 October 2018
    Published: 27 November 2018
    Abstract: Recently, we have established a novel assay to detect interaction of RNA-binding proteins (RBPs) with RNA, using biotinylated RNA oligos to capture RBPs with Western blot of specific antibodies against RBPs. The assay detects RNA binding more confidently than the traditional gel shift assay. Starting with completely randomized RNA oligos from 5mer ... Show More