Research Article
Analysis and Validation of Autophagy Related Genes in Septic Acute Kidney Injury
Pan Yang
,
Chen Si*
Issue:
Volume 11, Issue 2, June 2025
Pages:
24-35
Received:
24 July 2025
Accepted:
5 August 2025
Published:
26 August 2025
Abstract: Background: The pathogenesis of acute kidney injury (AKI) in sepsis involves inflammatory response. Autophagy has been shown to regulate inflammatory response, but the role of autophagy-related genes (ARGs) in the regulation of inflammation in septic AKI requires further investigation. Methods: Initially, the dataset GSE57065 was downloaded and utilized in the R language to investigate differentially expressed autophagy-related genes (DEARGs) associated with septic shock. Subsequently, DEARGs enrichment analysis and protein-protein interactions (PPIs) were conducted. Hub genes were identified through PPIs, and their diagnostic value was evaluated using ROC analyses with the external dataset GSE65682. Additionally, the septic AKI animal model was established to validate hub genes through qRT-PCR. Finally, immune cell infiltration in the septic AKI and control group was analyzed. Furthermore, we examined the correlation between immune cell infiltration and the validated hub genes, and predicted the miRNA-mRNA network. Results: In the GSE57065 dataset, we have identified 22 differentially expressed genes (DEGs) primarily involved in autophagy. The receiver operating characteristic (ROC) analysis suggested that the top ten hub genes may have diagnostic value. In our animal experiment, qRT-PCR results demonstrated elevated expressions of TP53, MYC, FOXO1, CXCR4, and BCL-2, while PARP1 and PTEN exhibited reduced expressions in septic AKI. The CIBERSORT analysis revealed immune infiltration, and the validated hub genes were associated with immune cell infiltration in septic AKI. Lastly, we have discovered potential regulatory miRNAs through the miRNA-mRNA network. Conclusions: The potential diagnostic and therapeutic targets, namely TP53, MYC, FOXO1, CXCR4, BCL-2, PARP1, and PTEN, have been identified as potentially influential factors in the infiltration of immune cells in acute kidney injury associated with sepsis.
Abstract: Background: The pathogenesis of acute kidney injury (AKI) in sepsis involves inflammatory response. Autophagy has been shown to regulate inflammatory response, but the role of autophagy-related genes (ARGs) in the regulation of inflammation in septic AKI requires further investigation. Methods: Initially, the dataset GSE57065 was downloaded and uti...
Show More
Research Article
Perspective of Prophetic and Islamic Medicine in Sokoto Caliphate and Their Potentials to Modern Medicine
Mukhtar Umar Dagimun*
Issue:
Volume 11, Issue 2, June 2025
Pages:
36-41
Received:
20 December 2024
Accepted:
13 January 2025
Published:
29 August 2025
Abstract: The noble Scholars of Sokoto caliphate had left an important legacy on Prophetic and Islamic medicine which is evidenced in a number of works they had written. They realized the significance of health to the life of the people and hence produced valuable literature on medicine. The objective of this research paper is to analyze some of these literary works of the Jihad Scholars contextually and show the potential of the knowledge to the field of human medicine. Despite the significance of these works by the Jihad Scholars, yet they have not been given due regards and consideration in our contemporary period. To achieve the objective of this research, analytical method and content analysis of some relevant works on medicine were employed. Some of the results of the findings show that a number of these medicinal literature are very much relevant in the modern field of human medicine. In view of this, there is need for both the government and other relevant stakeholders to come in, support and provide sponsor for the proper utilization of this legacy with a view to augmenting the health policies in the society particularly by both the government and other non-governmental organizations. The paper provides an insightful analysis of the contributions of Sokoto Jihad scholars to the field of human medicine, emphasizing their relevance to modern medical practices. It highlights the works of the Jihad Scholars across various branches of medicine, including pharmacology, surgery, community health, and epidemiology. The paper emphasizes for the contemporary relevance of these works, suggesting their integration into modern educational curricula, among others.
Abstract: The noble Scholars of Sokoto caliphate had left an important legacy on Prophetic and Islamic medicine which is evidenced in a number of works they had written. They realized the significance of health to the life of the people and hence produced valuable literature on medicine. The objective of this research paper is to analyze some of these litera...
Show More
