Volume 4, Issue 1, March 2018, Page: 1-9
Evaluation of Biofield Energy Treated Vitamin D3 in Human Osteoblasts Cells
Vicki Lynn Kindy, Trivedi Global, Inc., Henderson, USA
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, USA
Alice Branton, Trivedi Global, Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Received: Dec. 4, 2017;       Accepted: Jan. 11, 2018;       Published: Jan. 22, 2018
DOI: 10.11648/j.bs.20180401.11      View  1719      Downloads  100
Abstract
Bone formation, remodeling and healing processes involves a coordinated action of different cell types. Vitamin D and calcium significantly improves the patients' quality of life with respect to various bone disorders and comorbidities most prevalent in the elderly. The current study aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Vicki Lynn Kindy and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. Cell viability using 3-(4, 5-diamethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium) (MTT) data showed that the test items were found to be safe. ALP level was significantly increased by 621.2%, 638.9%, and 102.2% at 1, 10, and 50 µg/mL, respectively in UT-DMEM+BT-TI, as compared to the untreated group. Collagen content was significantly increased up to 284.6%, 134%, and 183.4% at 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 109.6% and 159.6% increased collagen in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 1 µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 120% and 229.4% at 50 and 100 µg/mL, respectively in UT-DMEM+BT-TI group, while 254.7% and 78.1% in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 10 µg/mL as compared with the untreated group. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.
Keywords
The Trivedi Effect®, Bone Disorders, Osteosarcoma Cells (MG-63), Alizarin Red S Staining, ALP, Collagen, Bone Mineralization
To cite this article
Vicki Lynn Kindy, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana, Evaluation of Biofield Energy Treated Vitamin D3 in Human Osteoblasts Cells, Biomedical Sciences. Vol. 4, No. 1, 2018, pp. 1-9. doi: 10.11648/j.bs.20180401.11
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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