Volume 3, Issue 3, May 2017, Page: 63-66
Classical Methods of Tissue Studies: The Artifacts and the Impact of Transverse Serial Sections
DEH Zhou Patricia, Laboratory of Histology-Embryology-Cytogenetic, UFR-Medical Sciences-University Felix Houphouēt-Boigny, Abidjan, Côte d’Ivoire
Koffi DP, Department of Endocrinology-Diabetology, University Teaching Hospital of Yopougon, Abidjan, Côte d’Ivoire
Atto V, Laboratory of Animal Physiology, UFR-Biosciences-University Felix Houphouēt-Boigny, Abidjan, Côte d’Ivoire
Monteomo GF, Laboratory of Animal Physiology, Pharmacology-Pharmacopoeia, UFR-Nature Science-University Nangui-Abrogoua, Abidjan, Côte d’Ivoire
Received: May 26, 2017;       Accepted: Jul. 19, 2017;       Published: Aug. 9, 2017
DOI: 10.11648/j.bs.20170303.12      View  1787      Downloads  160
Abstract
This work aims to study the mechanism of appearance of the histological alterations inherent in classical methods of tissue study and to give the difficulties related to the incidence of transverse serial sections. 4 lefts and rights testes were taken from two immature rats aged between 11 and 12 days. The testes were taken under anesthesia by ether inhalation. The gonads were fixed in 10% formic aldehyde and dehydrated in alcohol and then included in the paraffin. The ribbon of transverse serial sections of 5 μm were made by a microtome from the upper pole to the lower pole of the testes. The portions of the ribbon were then spread on slides. The smears were stained with hemalun-eosin and then covered with coverslip. The observation of transverse serial sections presented different types of degradation on them caused by technology. This involves deforming the contours of sections characterized by dye deposits, albuginea folds, tearing or destruction of the seminiferous tubes and then the formation of artificial vacuum between the tubes in the testicular parenchyma. It resulted from the stage of section, the loss of the three-dimensional (3D) coherence of the testes, generating the images visualized in a plan in a two-dimensional (2D) plane resulting from the serial histological sections. The results of this study show that the steps of the methods of tissue study are major factors that can condition the success or failure of reading the results of the histological technique. This study invites us to consider artefacts that combine artificial images created by classical methods of tissue study. In addition, images visualized in a plan with two dimensions (2D) do not allow a direct assessment of the spatial configuration. Thus, a better understanding of the structures, a correction of deformities and a three-dimensional reconstitution (3D) could improve compliance.
Keywords
Artefacts, Serial Sections, Incidence of the Sections, Optical Microscope, Wistar Rats
To cite this article
DEH Zhou Patricia, Koffi DP, Atto V, Monteomo GF, Classical Methods of Tissue Studies: The Artifacts and the Impact of Transverse Serial Sections, Biomedical Sciences. Vol. 3, No. 3, 2017, pp. 63-66. doi: 10.11648/j.bs.20170303.12
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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