Volume 3, Issue 3, May 2017, Page: 58-62
Perpetual Smoking Compromise the Osmotic Fragility of RBC
Mazhar Mushtaq, Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Uzma Jabbar, Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Mumtaz Begum, Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Naim Ahmad Nazami, Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Received: May 16, 2017;       Accepted: Jun. 14, 2017;       Published: Jul. 21, 2017
DOI: 10.11648/j.bs.20170303.11      View  1662      Downloads  135
Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.
Hydrogen Peroxide, Reactive Oxygen Species, Oxidative Stress, Catalase, Glutathione, Smoker
To cite this article
Mazhar Mushtaq, Uzma Jabbar, Mumtaz Begum, Naim Ahmad Nazami, Perpetual Smoking Compromise the Osmotic Fragility of RBC, Biomedical Sciences. Vol. 3, No. 3, 2017, pp. 58-62. doi: 10.11648/j.bs.20170303.11
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Veal E, Day A. (2011). Hydrogen peroxide as a signaling molecule. Antioxid Redox Signal. 1:147-151.
Loo AEKiat, Wong YT, Ho R, Wasser M, Tiehua D, Wee T. et al. (2012) Effects of Hydrogen Peroxide on Wound Healing in Mice in Relation to Oxidative Damage. PLoS ONE. 11: e49215.
Umit MS, Esra B, Serpil E, Cansin S, Omer K. (2011) Oxidative Stress in Asthma. World Allergy Organization. 4: 151–158.
Fernando H. (2013) Oxidative Stress in Airway Diseases. Ann Am Thorac Soc. 10: S150–S157.
Reinskje T, Thomas S, Ewa F, Jan-van B, Piet W, Antoon O. (2011) Hazardous Compounds in Tobacco Smoke. Int J Environ Res Public Health. 2: 613–628.
Rockville. How Tobacco Smoke Causes Disease: (2010) The Biology and Behavioral Basis for Smoking-Attributable Disease. U.S. Department Of Health And Human Services. http://www.surgeongeneral.gov/library.
Aydogan U, Durmaz E, Ercan CM, Eken A, Ulutas OK, et al. (2013) Smoking during pregnancy, DNA damage and ROS level. Arh Hig Rada Toksikol. 64: 35-46.
Jiayuan Z, Philip KH. (2012) Concentration of Reactive Oxygen Species (ROS) in Mainstream and Side-stream Cigarette Smoke. Aerosol Science and Technology. 2: 191-197.
Zhao-Li C, Jian T, Jie Y, Zhen-Li Y, Xing-Hua L, Min J, et al. (2011) Vitamin E Modulates Cigarette Smoke Extract-induced Cell Apoptosis in Mouse Embryonic Cells. Int J Biol Sci. 7: 927-936.
Corinne CW, Claudia PP, Peter G, Florence V, Gabriele S, et al. (2010) Hemoglobin can attenuate hydrogen peroxide–Induced oxidative stress by acting as an antioxidative peroxidase. Antioxid Redox Signal. 2: 185–198.
Pandey KB, Rizvi SI. (2011) Biomarkers of oxidative stress in red blood cells. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2: 131–136.
Mahapatra SK, Das S, Dey SK, Roy S. (2008) Smoking induced oxidative stress in serum and neutrophil of the university students. Al Ameen J Med Sci. 1: 20-31.
Kosmider B, Messier EM, Chu HW, Mason RJ. (2011) Human Alveolar Epithelial Cell Injury Induced by Cigarette Smoke. PLoS ONE. 12: e26059.
Betsuyaku T, Fuke S, Inomata T, Kaga K, Morikawa T, Odajima N. et al. (2013) Bronchiolar epithelial catalase is diminished in smokers with mild COPD. Eur Respir J. 42: 42–53.
Biju T, Amitha R, Sneha S, Rajendra P. (2013) A comparative evaluation of antioxidant enzymes and selenium in the serum of periodontitis patients with DM type 2. Contemporary clinical dentistry. 4: 176–180.
Neal SG, Elysia M, Steve G, Richard JM, Brian JD. (2011) Lung glutathione adaptive responses to cigarette smoke exposure. Respiratory Research. 12: 133-142.
Yousif YB, Sanaa S, Talal A, Shtywy A. (2012) Structure activity relationships regarding the antioxidant effects of the flavonoids on human erythrocytes. Natural Science. 4: 740–747.
Mohamed J, Shing SW, Idris MH, Budin SB, Zainalabidin S. (2013). The protective effect of aqueous extracts of roselle against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes. Clinics. 68: 1358-1363.
Abbas M, Ali R. (2013) Erythrocyte osmotic fragility test revealed protective effects of supplementation with saffron and cinnamon on the Red Blood Cell membrane. Asian J. exp. biol. sci. 2: 322-326.
Ergueder, Imge B., et al. (2009) The effects of cigarette smoking on serum oxidant status, and cholesterol, homocysteine, folic acid, copper, and zinc levels in university students. Turkish Journal of Medical Sciences. 4: 513-517.
Padmavathi, Pannuru, Vaddi Damodara Reddy, and Nallanchakravarthula Varadacharyulu. (2009) Influence of chronic cigarette smoking on serum biochemical profile in male human volunteers. Journal of Health science. 2: 265-270.
Cano, Marisol, et al. (2010) Cigarette smoking, oxidative stress, the anti-oxidant response through Nrf2 signaling, and Age-related Macular Degeneration. Vision research. 7: 652-664.
Rao, P. S. S., Ande, A., Sinha, N., Kumar, A., & Kumar, S. (2016). Effects of cigarette smoke condensate on oxidative stress, apoptotic cell death, and HIV replication in human monocytic cells. PloS one. 5: e0155791.
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