Volume 3, Issue 3, May 2017, Page: 58-62
Perpetual Smoking Compromise the Osmotic Fragility of RBC
Mazhar Mushtaq, Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Uzma Jabbar, Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Mumtaz Begum, Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Naim Ahmad Nazami, Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Received: May 16, 2017;       Accepted: Jun. 14, 2017;       Published: Jul. 21, 2017
DOI: 10.11648/j.bs.20170303.11      View  1662      Downloads  135
Abstract
Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.
Keywords
Hydrogen Peroxide, Reactive Oxygen Species, Oxidative Stress, Catalase, Glutathione, Smoker
To cite this article
Mazhar Mushtaq, Uzma Jabbar, Mumtaz Begum, Naim Ahmad Nazami, Perpetual Smoking Compromise the Osmotic Fragility of RBC, Biomedical Sciences. Vol. 3, No. 3, 2017, pp. 58-62. doi: 10.11648/j.bs.20170303.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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